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A simple screening procedure for adenylate kinase, hexokinase and glucose-6-phosphate dehydrogenase deficiencies.

Authors
  • Vaca, G
  • Sanchez-Corona, J
  • Olivares, N
  • Medina, C
  • Cantu, J M
Type
Published Article
Journal
Annales de génétique
Publication Date
Jan 01, 1980
Volume
23
Issue
3
Pages
190–192
Identifiers
PMID: 6252822
Source
Medline
License
Unknown

Abstract

A simple screening procedure for the detection of adenilate kinase (AK), hexokinase (Hx) or glucose-6-phosphate dehydrogenase (G6PD) deficiencies in blood, is described. It consists of two assays : in the first, the ATP formed by blood AK is coupled to Hx and G6PD, and in the second, the glucose-6-phosphate formed by blood Hx is coupled to G6PD. The enzyme activities are visually estimated by the reduction of NADP+ (non-fluorescent) to NADH (fluorescent). The appearance of fluorescence in the first assay indicates that the three enzyme activities are present. The absence of fluorescence could be due to the deficiency of any one of the three enzymes; in this case the second assay used in combination with the Beutler's screening test for G6PD permits the detection of the specific enzymatic deficiency.

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