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A simple radioimmunoassay for plasma cortisol and 11-deoxycortisol (17, 21-dihydroxy-4-pregnene-3, 20-dione).

Authors
  • Brown, J R
  • Cavanaugh, A H
  • Farnsworth, W E
Type
Published Article
Journal
Steroids
Publisher
Elsevier
Publication Date
Oct 01, 1976
Volume
28
Issue
4
Pages
487–498
Identifiers
PMID: 1034356
Source
Medline
License
Unknown

Abstract

A simple procedure for the measurement of cortisol and 11-deoxycortisol in plasma or serum is described. One-half ml. of plasma is extracted with methylene chloride. Separation of cortisol and deoxycortisol is achieved by a Sephadex LH-20 mini-column. The assay itself is achieved by the use of a commercially available cortisol kit employing rabbit anti-cortisol coated tubes. This antibody exhibits a 20% crossreactivity with 11-deoxycortisol. This procedure is extremely useful in the assessment of a pituitary-adrenal reserve in the Metyrapone test.

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