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A simple purification of calmodulin by reversed phase chromatography with hydrophobic polymer 3520.

Authors
  • Ho, A K
  • Ling, Q L
  • Shang, K J
  • Li, C F
  • Wang, L C
Type
Published Article
Journal
Preparative biochemistry
Publication Date
Jan 01, 1986
Volume
16
Issue
4
Pages
297–308
Identifiers
PMID: 3101057
Source
Medline
License
Unknown

Abstract

A new adsorption chromatography procedure for the purification of calmodulin from bovine brain was developed using polymeric adsorbent 3520. Calmodulin was first isolated by DEAE-Cellulose column chromatography and further purified to apparent homogeneity following elution with 50% ethanol from the adsorbent column. Polyacrylamide gel electrophoresis showed one band either in the presence of Ca2+ or EGTA. The polymeric adsorbent 3520 is a non-polar polymer lacking exchangeable groups. The selective adsorption of calmodulin is based on hydrophobic interaction within the matrix, and is Ca2+ independent. Neither high salt (0.5 M NaC1) nor EGTA (5 mM) was able to elute the CaM from the adsorption column whereas ethanol (50%) eluted it completely. This method is simple to use and it provides highly purified calmodulin with high yield.

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