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A simple procedure for the determination of the trapped volume of liposomes

Authors
  • Oku, Naoto
  • Kendall, Debra A.
  • MacDonald, Robert C.
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier
Publication Date
Jan 01, 1982
Volume
691
Issue
2
Pages
332–340
Identifiers
DOI: 10.1016/0005-2736(82)90422-9
Source
Elsevier
Keywords
License
Unknown

Abstract

A new method is described for determining the volume of the aqueous compartment of liposomes. Liposomes are prepared in a solution of the fluorescent dye, calcein. The fraction of the total volume that is within the liposomes is obtained as the fraction of the fluorescence that remains after adding cobalt(II) ions which, when chelated by calcein, quench its fluorescence. The method is rapid, simple and accurate. Separation of the liposomes from the medium is not required. The procedure is equally well suited to the assay of permeability characteristics of liposomal membranes.

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