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A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids.

Authors
  • Brian, B L
  • Gardner, E W
Type
Published Article
Journal
Applied microbiology
Publication Date
Apr 01, 1968
Volume
16
Issue
4
Pages
549–552
Identifiers
PMID: 4869615
Source
Medline
License
Unknown

Abstract

Four gram-negative bacterial species, including Escherichia coli strain B, Serratia marcescens, Pseudomonas fluorescens, and Vibrio cholerae (comma) strain NIH 41, were investigated for fatty acid content by gas-liquid chromatography involving a preparatory technique which facilitated detection of cyclopropane fatty acids. Methyl esters of fatty acids were subjected to mild catalytic hydrogenation to eliminate unsaturates. Hydrogenation was followed by bromination which removed cyclopropane acids from chromatographic profile patterns. Lactobacillic acid (cis-11,12-methyleneoctanoate) and cis-9,10-methylenehexadecanoate, previously reported in lipids of E. coli and S. marcescens, were found in small amounts in P. fluorescens but were not detected in V. cholerae.

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