Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.
Department of Chemistry, University of Hull, Cottingham Road, Hull, UK HU6 7RX.
- Published Article
Lab on a Chip
The Royal Society of Chemistry
- Publication Date
Dec 07, 2009
A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.
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This record was last updated on 06/09/2018 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/19904411