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Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.

Authors
  • Shaw, Kirsty J1
  • Joyce, Domino A
  • Docker, Peter T
  • Dyer, Charlotte E
  • Greenman, John
  • Greenway, Gillian M
  • Haswell, Stephen J
  • 1 Department of Chemistry, University of Hull, Cottingham Road, Hull, UK HU6 7RX.
Type
Published Article
Journal
Lab on a Chip
Publisher
The Royal Society of Chemistry
Publication Date
Dec 07, 2009
Volume
9
Issue
23
Pages
3430–3432
Identifiers
DOI: 10.1039/b913309g
PMID: 19904411
Source
Medline
License
Unknown

Abstract

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was successfully extracted from buccal swabs using electro-osmotic pumping (EOP) coupled with in situ reagents contained within a 1.5% agarose gel matrix. The extracted DNA was of sufficient quantity and purity for polymerase chain reaction (PCR) amplification.

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