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A simple method for short-term maintenance of neonatal mice without foster mothers

Authors
  • Kreikemeier-Bower, Craig1
  • Polepole, Pascal2
  • Pinkerton, Katherine1
  • Zhang, Luwen2, 3
  • 1 Institution of Animal Care Program, University of Nebraska, Lincoln, NE 68583, USA
  • 2 Nebraska Center for Virology, University of Nebraska, Lincoln, NE 68583, USA
  • 3 School of Biological Sciences, University of Nebraska, Lincoln, NE 68583, USA
Type
Published Article
Journal
Journal of Biological Methods
Publisher
Journal of Biological Methods
Publication Date
Feb 17, 2020
Volume
7
Issue
1
Identifiers
DOI: 10.14440/jbm.2020.315
PMID: 32201708
PMCID: PMC7081055
Source
PubMed Central
Keywords
License
Unknown

Abstract

Mice are typically weaned from their mother between 21 and 28 days of age, or at 10 grams of body weight. However, some biochemical experiments need to be done before the weaning days, and the mother might cannibalize or ignore those manipulated pups. Here, we provide a detailed protocol for maintenance of neonatal mice without the presence of their mothers for biomedical research. The basic instinct of neonate mice to hide under covers is harnessed for their survival in a mother-free environment. When covers are soaked with milk and the only targets for hiding, the neonates would acquire their nutrients at least in an involuntary fashion. The protocol is simple and can be used for neonatal rodent studies for short periods of times, and assures the accuracy of the biomedical experiments if survival rate of neonates is critical.

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