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A simple method of isolation of chloramphenicol in honey and its estimation by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

Authors
Type
Published Article
Journal
Rapid Communications in Mass Spectrometry
0951-4198
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
19
Issue
21
Pages
3025–3030
Identifiers
PMID: 16193535
Source
Medline

Abstract

To detect sub-ppb levels of the antibiotic chloramphenicol in honey matrix, a convenient method of extraction and measurement using liquid chromatography with detection by tandem mass spectrometry (LC/MS/MS) was developed. Honey samples fortified with chloramphenicol and isotopically labeled chloramphenicol were extracted using diatomaceous-based supported liquid-liquid extraction cartridges to generate a standard calibration curve. Four MS/MS transitions were used for quantification and four other transitions for confirmation of chloramphenicol. The limit of detection for chloramphenicol was 0.05 ng/g and the lower limit of quantification was 0.1 ng/g. Several commercial honey samples were analyzed for chloramphenicol content using this method.

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