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A simple method of isolation of chloramphenicol in honey and its estimation by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.

Authors
  • Vivekanandan, Kannan
  • Swamy, Madugundu Guru
  • Prasad, Sudhanand
  • Mukherjee, Rama
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Jan 01, 2005
Volume
19
Issue
21
Pages
3025–3030
Identifiers
PMID: 16193535
Source
Medline
License
Unknown

Abstract

To detect sub-ppb levels of the antibiotic chloramphenicol in honey matrix, a convenient method of extraction and measurement using liquid chromatography with detection by tandem mass spectrometry (LC/MS/MS) was developed. Honey samples fortified with chloramphenicol and isotopically labeled chloramphenicol were extracted using diatomaceous-based supported liquid-liquid extraction cartridges to generate a standard calibration curve. Four MS/MS transitions were used for quantification and four other transitions for confirmation of chloramphenicol. The limit of detection for chloramphenicol was 0.05 ng/g and the lower limit of quantification was 0.1 ng/g. Several commercial honey samples were analyzed for chloramphenicol content using this method.

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