Affordable Access

A simple immunometric assay to assess the feeding habits of Meprai spinolai, a Trypanosoma cruzi vector.

Authors
  • Molina, María C
  • Cattán, Pedro
  • Canals, Mauricio
  • Cruzat, Loreto
  • Aguillón, Juan C
  • Ferreira, Arturo
Type
Published Article
Journal
Parasitology research
Publication Date
Mar 01, 2004
Volume
92
Issue
5
Pages
375–379
Identifiers
PMID: 14745546
Source
Medline
License
Unknown

Abstract

We propose a simple assay to assess the importance of seven vertebrate species as food sources for Mepria spinolai, a wild arthropod vector of Trypanosoma cruzi (the agent of Chagas' disease). Rabbits were immunized with serum proteins from one of each of the seven species. After titration, a consensus 1/100,000 dilution of the immune sera detected vertebrate serum proteins in the intestinal contents of 48.9% of 131 insects tested. The high proportion of negative samples is consistent with previous information indicating that these insects can withstand prolonged fasting periods. Alternatively, they may have fed on a different animal species than those used to produce the antisera. In about 70% of the positive samples, only one species of serum protein was detected. All pre-immune sera were negative. In 67% of the positive vectors, rabbit immunoglobulins were detected directly by means of a specific goat antibody. Thus, rabbits may play a role in T. cruzi transmission.

Report this publication

Statistics

Seen <100 times