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A simple high-performance liquid chromatography (HPLC) method for the measurement of pyridoxal-5-phosphate and 4-pyridoxic acid in human plasma.

Authors
  • Cabo, Rona1
  • Kozik, Karolina2
  • Milanowski, Maciej2
  • Hernes, Sigrunn3
  • Slettan, Audun1
  • Haugen, Margaretha4
  • Ye, Shu5
  • Blomhoff, Rune6
  • Mansoor, M Azam7
  • 1 Department of Natural Sciences, University of Agder, Kristiansand, Norway. , (Norway)
  • 2 Department of Biochemistry, University of Technology and Life Sciences, Bydgoszcz, Poland. , (Poland)
  • 3 Department of Nutrition and Public Health, University of Agder, Kristiansand, Norway. , (Norway)
  • 4 National Institute of Public Health, Oslo, Norway. , (Norway)
  • 5 William Harvey Research Institute, Queen Marry University of London, London, UK.
  • 6 Department of Nutrition, University of Oslo, Olso, Norway; Division of Cancer Medicine, Surgery and Transplantation, Oslo University Hospital, Olso, Norway. , (Norway)
  • 7 Department of Natural Sciences, University of Agder, Kristiansand, Norway; Department of Nutrition, University of Oslo, Olso, Norway. Electronic address: [email protected] , (Norway)
Type
Published Article
Journal
Clinica chimica acta; international journal of clinical chemistry
Publication Date
Jun 10, 2014
Volume
433
Pages
150–156
Identifiers
DOI: 10.1016/j.cca.2014.03.003
PMID: 24657184
Source
Medline
Keywords
License
Unknown

Abstract

The present method is suitable for large clinical studies for the measurement of plasma PLP and 4-PA. Our findings demonstrate that plasma 4-PA, BMI and sex are the major determinants of plasma PLP in healthy individuals.

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