A simple fixation and embedding method for use in hybridization histochemistry on plant tissues.
- Published Article
The Histochemical journal
- Publication Date
Oct 01, 1988
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/2464563