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A simple fixation and embedding method for use in hybridization histochemistry on plant tissues.

Authors
  • McFadden, G I
  • Bonig, I
  • Cornish, E C
  • Clarke, A E
Type
Published Article
Journal
The Histochemical journal
Publication Date
Oct 01, 1988
Volume
20
Issue
10
Pages
575–586
Identifiers
PMID: 2464563
Source
Medline
License
Unknown

Abstract

A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.

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