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A simple enzymic method for the synthesis of [32P]phosphoenolpyruvate.

Authors
  • Parra, F
Type
Published Article
Journal
The Biochemical journal
Publication Date
Sep 01, 1982
Volume
205
Issue
3
Pages
643–645
Identifiers
PMID: 7150238
Source
Medline
License
Unknown

Abstract

A rapid and simple enzymic method is described for the synthesis of [(32)P]phosphoenolpyruvate from [(32)P]P(i), with a reproducible yield of 74%. The final product was shown to be a good substrate for pyruvate kinase (EC 2.7.1.40).

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