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A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord

Authors
  • Mishra, Pooja Shree
  • Raju, Trichur R
Type
Published Article
Journal
Bio-protocol
Publication Date
Jan 20, 2020
Volume
10
Issue
2
Identifiers
DOI: 10.21769/BioProtoc.3501
PMID: 33654728
PMCID: PMC7842766
Source
PubMed Central
Keywords
License
Unknown

Abstract

Investigations into glial biology have contributed substantially in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo present significant challenges while delineating the individual cell-type contributions, thus making the in vitro techniques exceedingly relevant to study glial biology. However, obtaining optimal yield along with high purity has been challenging for microglial cultures. Here we present a simple protocol to establish enriched astroglial as well as microglial cultures from the neonatal rat spinal cord. This method results in highly enriched astroglial and microglial cultures with maximal yield.

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