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A simple and economical DRB1 typing procedure combining group-specific amplification, DNA heteroduplex and enzyme restriction analysis.

Authors
  • D'Amato, M
  • Sorrentino, R
Type
Published Article
Journal
Tissue antigens
Publication Date
May 01, 1994
Volume
43
Issue
5
Pages
295–301
Identifiers
PMID: 7940498
Source
Medline
License
Unknown

Abstract

A new procedure for HLA-DRB1 typing is proposed. The method combines six group-specific amplifications with heteroduplex analysis and, in some cases, enzyme restriction analysis. This technique, which is as discriminative as oligotyping, is simple, economical and does not require probes. These characteristics make this approach a valid alternative to other HLA genomic typing procedures, especially in those cases such as donor-recipient pairs matching where a small number of samples has to be managed at once.

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