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A simple apparatus for electrokinetic removal of sodium dodecyl sulfate from protein digests.

Authors
  • Pandigamage, Pravinda1
  • Wilson, Richard2
  • Quirino, Joselito P3
  • 1 Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Hobart 7001, Australia. , (Australia)
  • 2 Central Science Laboratory, Research Division, University of Tasmania, Hobart 7001, Australia. , (Australia)
  • 3 Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences-Chemistry, University of Tasmania, Hobart 7001, Australia. Electronic address: [email protected] , (Australia)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Sep 27, 2020
Volume
1628
Pages
461443–461443
Identifiers
DOI: 10.1016/j.chroma.2020.461443
PMID: 32822982
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Sodium dodecyl sulfate (SDS) in proteomics samples needs to be removed and estimated prior to mass spectrometry (MS)-based analysis and to avoid MS ion-source contamination. Here, we describe an organic solvent free method to remove SDS using a simple apparatus that mainly consists of an agarose gel inside a 1 mL plastic micropipette tip and a voltage power supply with electrodes. A small volume of sample (e.g., 50 μL) is loaded on top of the gel and then voltage (cathode at the sample side) is applied with an acidic solution at the other end of the micropipette tip. Within 25 min, SDS was removed (e.g., ≥99% SDS in 3.5 mM SDS) and the peptides were retained in the sample solution. The strategy was compared to the commercially available and expensive Pierce spin column for the removal of SDS and recovery of peptides from a digested bovine serum albumin sample. Copyright © 2020. Published by Elsevier B.V.

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