Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Affordable Access

Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

Publication Date
Sep 01, 1983
Source
PMC
Keywords
License
Unknown

Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

Report this publication

Statistics

Seen <100 times