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Silk based bioinks for soft tissue reconstruction using 3-dimensional (3D) printing with in vitro and in vivo assessments.

Authors
  • Rodriguez, María J1
  • Brown, Joseph1
  • Giordano, Jodie1
  • Lin, Samuel J2
  • Omenetto, Fiorenzo G1
  • Kaplan, David L3
  • 1 Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
  • 2 Division of Plastic and Reconstructive Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA. , (Israel)
  • 3 Department of Biomedical Engineering, Tufts University, Medford, MA, USA. Electronic address: [email protected]
Type
Published Article
Journal
Biomaterials
Publication Date
Feb 01, 2017
Volume
117
Pages
105–115
Identifiers
DOI: 10.1016/j.biomaterials.2016.11.046
PMID: 27940389
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In the field of soft tissue reconstruction, custom implants could address the need for materials that can fill complex geometries. Our aim was to develop a material system with optimal rheology for material extrusion, that can be processed in physiological and non-toxic conditions and provide structural support for soft tissue reconstruction. To meet this need we developed silk based bioinks using gelatin as a bulking agent and glycerol as a non-toxic additive to induce physical crosslinking. We developed these inks optimizing printing efficacy and resolution for patient-specific geometries that can be used for soft tissue reconstruction. We demonstrated in vitro that the material was stable under physiological conditions and could be tuned to match soft tissue mechanical properties. We demonstrated in vivo that the material was biocompatible and could be tuned to maintain shape and volume up to three months while promoting cellular infiltration and tissue integration. Copyright © 2016 Elsevier Ltd. All rights reserved.

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