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Silibinin Promotes Cell Proliferation Through Facilitating G1/S Transitions by Activating Drp1-Mediated Mitochondrial Fission in Cells.

Authors
  • You, Yanting1, 2
  • Chen, Liqian2
  • Wu, Yifen3
  • Wang, Ming1
  • Lu, Hanqi2
  • Zhou, Xinghong2
  • Liu, Huaxi2
  • Fu, Zixuan2
  • He, Qiuxing2
  • Ou, Jinying4
  • Fu, Xiuqiong5
  • Liu, Yanyan1
  • Kwan, Hiuyee5
  • Liang, Donghui1
  • Zhao, Xiaoshan2
  • Dai, Jiaojiao2
  • 1 Department of Traditional Chinese Medicine, 70570Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China. , (China)
  • 2 Syndrome Laboratory of Integrated Chinese and Western Medicine, School of Chinese Medicine, 70570Southern Medical University, Guangzhou, Guangdong, China. , (China)
  • 3 Department of Oncology, Affiliated Dongguan People's Hospital, 70570Southern Medical University, Guangzhou, Guangdong, China. , (China)
  • 4 Traditional Chinese Pharmacological Laboratory, School of Traditional Chinese Medicine, 70570Southern Medical University, Guangzhou, Guangdong, China. , (China)
  • 5 School of Chinese Medicine, 26679Hong Kong Baptist University, Hong Kong, China. , (China)
Type
Published Article
Journal
Cell Transplantation
Publisher
SAGE Publications
Publication Date
Jan 01, 2020
Volume
29
Identifiers
DOI: 10.1177/0963689720950213
PMID: 32830544
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Heart, liver, and kidney, which are known as the essential organs for metabolism, possess the unique ability to regulate the proliferation function of the body against injury. Silibinin (SB), a natural polyphenolic flavonoid extracted from traditional herb Silybum marianum L., has been used to protect hepatocytes. Whether SB can regulate mitochondrial fission in normal cells and the underlying mechanisms remain unclear. Here, we showed that SB markedly promoted cell proliferation by facilitating G1/S transition via activating dynamin-related protein 1 (Drp1), which in turn mediated mitochondrial fission in these normal cells. SB dose-dependently increased the mitochondrial mass, mtDNA copy number, cellular adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species in normal cells. Furthermore, SB dose-dependently increased the expression of Drp1. Blocking Drp1 abolished SB-induced mitochondrial fission. In conclusion, we demonstrate that SB promotes cell proliferation through facilitating G1/S transition by activating Drp1-mediated mitochondrial fission. This study suggests that SB is a potentially useful herbal derivative for the daily prevention of various diseases caused by impaired mitochondrial fission.

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