SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective.
- Authors
- Publication Date
- Jan 01, 2017
- Source
- Apollo - University of Cambridge Repository
- Keywords
- Language
- English
- License
- Unknown
- External links
Abstract
Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.
