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Signaling lipids as diagnostic biomarkers for ocular surface cicatrizing conjunctivitis

Authors
  • Di Zazzo, Antonio1
  • Yang, Wei2
  • Coassin, Marco1
  • Micera, Alessandra3
  • Antonini, Marco1
  • Piccinni, Fabrizio1
  • De Piano, Maria3
  • Kohler, Isabelle4
  • Harms, Amy C.2
  • Hankemeier, Thomas2
  • Boinini, Stefano1
  • Mashaghi, Alireza2
  • 1 University Campus Bio-Medico, Rome, Italy , Rome (Italy)
  • 2 Leiden University, Leiden, 2333 CC, The Netherlands , Leiden (Netherlands)
  • 3 IRCCS Fondazione Bietti, Rome, Italy , Rome (Italy)
  • 4 Vrije Universiteit Amsterdam, Amsterdam, The Netherlands , Amsterdam (Netherlands)
Type
Published Article
Journal
Journal of Molecular Medicine
Publisher
Springer-Verlag
Publication Date
Apr 20, 2020
Volume
98
Issue
5
Pages
751–760
Identifiers
DOI: 10.1007/s00109-020-01907-w
Source
Springer Nature
Keywords
License
Green

Abstract

AbstractMetabolomics has been applied to diagnose diseases, predict disease progression, and design therapeutic strategies in various areas of medicine. However, it remains to be applied to the ocular surface diseases, where biological samples are often of limited quantities. We successfully performed proof-of-concept metabolomics assessment of volume-limited cytology samples from a clinical form of chronic inflammatory cicatrizing conjunctivitis, i.e., ocular MMP and discovered metabolic changes of signaling lipid mediators upon disease onset and progression. The metabolomics assessment revealed active oxylipins, lysophospholipids, fatty acids, and endocannabinoids alterations, from which potential biomarkers linked to inflammatory processes were identified. Possible underlying mechanisms such as dysregulated enzyme activities (e.g., lipoxygenases, cytochrome P450, and phospholipases) were suggested which may be considered as potential therapeutic targets in future studies.Key messagesMetabolic profile of the ocular surface can be measured using impression cytology samples.Metabolomics analysis of ocular pemphigoid is presented for the first time.The metabolomics assessment of OCP patients revealed active oxylipins, lysophospholipids, fatty acids, and endocannabinoids alterations.Several oxylipins are identified as diagnostic biomarkers for OCP.

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