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Signal to noise enhancement in dark field electron micrographs of vasopressin: filtering of arrays of images in reciprocal space.

Authors
  • Ottensmeyer, F P
  • Andrew, J W
  • Bazett-Jones, D P
  • Chan, A S
  • Hewitt, J
Type
Published Article
Journal
Journal of microscopy
Publication Date
Apr 01, 1977
Volume
109
Issue
3
Pages
259–268
Identifiers
PMID: 886609
Source
Medline
License
Unknown

Abstract

A technique for filtering arrays of images of dispersed molecules is presented which takes advantage of the crystallographic properties of regular arrays and the rapidity of optical treatment. A filter in reciprocal space consisting of perforations in a square or rectangular lattice, as determined by the image arrrangement, reduces noise and averages the images simultaneously by transmitting only regularly recurring image structure. The filter is universal for all image contents and introduces no additional biases. The signal to noise improvement approaches square root N for N independent images of the same molecule in the same configuration. The procedure which is simple, rapid and inexpensive, is demonstrated with the aid of dark field electron micrographs of the protein vasopressin and combined with iodination of that molecule to elucidate its structure.

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