Affordable Access

Signal-Mediated Depolymerization of Actin in Pollen during the Self-Incompatibility Response

Authors
Publisher
American Society of Plant Biologists
Publication Date
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Signal-Mediated Depolymerization of Actin in Pollen during the Self-Incompatibility Response by Benjamin N. Snowman, David R. Kovar, Galina Shevchenko, Vernonica E. Franklin-Tong, and Christopher J. Staiger Plantcell Volume 14(10):2613-2626 October 1, 2002 ©2002 by American Society of Plant Biologists Rearrangements of the Pollen F-Actin Cytoskeleton Are Triggered by SI.Alexa-488–phalloidin-stained pollen grains and tubes of poppy were imaged using confocal microscopy. Snowman B et al. Plantcell 2002;14:2613-2626 ©2002 by American Society of Plant Biologists The SI Response Induces F-Actin Depolymerization.The concentration of actin in the polymerized form in pollen grains and tubes was measured using the Alexa-488–phalloidin binding assay.(A) Pollen was grown in vitro for the times indicated and the amounts of... Snowman B et al. Plantcell 2002;14:2613-2626 ©2002 by American Society of Plant Biologists Drugs That Increase [Ca2+]i Result in Depolymerization of F-Actin.Pollen tubes were treated with drugs known to increase [Ca2+]i in poppy pollen tubes, and the concentration of actin in polymeric form was measured.(A) A23187 (50 μM; closed diamonds) stimula... Snowman B et al. Plantcell 2002;14:2613-2626 ©2002 by American Society of Plant Biologists Actin Organization Is Perturbed by Ca2+ Agonists.Pollen tubes were treated with drugs known to increase [Ca2+]i in poppy pollen tubes, and the F-actin cytoskeleton was imaged using fluorescent phalloidin. Snowman B et al. Plantcell 2002;14:2613-2626 ©2002 by American Society of Plant Biologists Viability of Pollen Is Retained during the Actin Depolymerization Process.The plot shows the percentage of pollen viability, as measured by fluorescein diacetate staining at 0, 10, 20, 40, and 60 min after treatment with incompatible S proteins. Snowman B et al. Plantcell 2002;14:2613-2626 ©2002 by American Society of Plant Biologists Profilin and Actin Antisera Recognize Poppy Proteins Specifically.Polyclonal antisera raised against maize pollen

Statistics

Seen <100 times