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Siglec-F is a novel intestinal M cell marker.

Authors
  • Gicheva, Nadezhda1
  • Macauley, Matthew S2
  • Arlian, Britni M3
  • Paulson, James C4
  • Kawasaki, Norihito5
  • 1 Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK.
  • 2 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
  • 3 Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.
  • 4 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA; Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: [email protected]
  • 5 Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK; Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA. Electronic address: [email protected]
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publisher
Elsevier
Publication Date
Oct 07, 2016
Volume
479
Issue
1
Pages
1–4
Identifiers
DOI: 10.1016/j.bbrc.2016.08.055
PMID: 27524237
Source
Medline
Keywords
License
Unknown

Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.

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