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Short-term effects of deoxynivalenol, T-2 toxin, fumonisin B1 or ochratoxin on lipid peroxidation and glutathione redox system and its regulatory genes in common carp ( Cyprinus carpio L.) liver

Authors
  • Kövesi, Benjámin1
  • Kulcsár, Szabina1
  • Zándoki, Erika2
  • Szabó-Fodor, Judit2
  • Mézes, Miklós1, 2
  • Balogh, Krisztián1, 2
  • Ancsin, Zsolt1
  • Pelyhe, Csilla1
  • 1 Szent István University,
  • 2 Hungarian Academy of Sciences-Kaposvár University- Szent István University, Mycotoxins in the Food Chain Research Group,
Type
Published Article
Journal
Fish Physiology and Biochemistry
Publisher
Springer-Verlag
Publication Date
Jul 02, 2020
Volume
46
Issue
6
Pages
1921–1932
Identifiers
DOI: 10.1007/s10695-020-00845-1
PMID: 32617788
PMCID: PMC7584534
Source
PubMed Central
Keywords
License
Unknown

Abstract

The effects of a single oral dose of 1.82 mg kg−1 bw of T-2 and HT-2 toxin (T-2), 1.75 mg kg−1 bw deoxynivalenol (DON) and 15-acetyl DON, 1.96 mg kg−1 bw fumonisin B1 (FB1) or 1.85 mg kg−1 bw ochratoxin A (OTA) were investigated in common carp juveniles on lipid peroxidation, the parameters of the glutathione redox system including the expression of their encoding genes in a short-term (24 h) experiment. Markers of the initiation phase of lipid peroxidation, conjugated dienes, and trienes, were slightly affected by DON and OTA treatment at 16-h sampling. The termination marker, malondialdehyde, concentration increased only as an effect of FB1. Glutathione content and glutathione peroxidase activity showed significantly higher levels in the T-2 and FB1 groups at 8 h, and in the DON and FB1 groups at 16 h. The expression of glutathione peroxidase genes ( gpx4a, gpx4b ) showed a dual response. Downregulation of gpxa was observed at 8 h, as the effect of DON, FB1, and OTA, but an upregulation in the T-2 group. At 16 h gpx4a upregulated as an effect of DON, T-2, and FB1, and at 24 h in the DON and T-2 groups. Expression of gpx4b downregulated at 8 h, except in the T-2 group, and upregulation observed as an effect of T-2 at 24 h. The lack of an increase in the expression of nrf2, except as the effect of DON at 8 h, and a decrease in the keap1 expression suggests that the antioxidant defence system was activated at gene and protein levels through Keap1–Nrf2 independent pathways.

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