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Short communication: Processed bovine colostrum milk protein concentrate increases epithelial barrier integrity of Caco-2 cell layers.

Authors
  • Anderson, Rachel C1
  • Dalziel, Julie E2
  • Haggarty, Neill W3
  • Dunstan, Kelly E4
  • Gopal, Pramod K5
  • Roy, Nicole C6
  • 1 Food Nutrition & Health Team, AgResearch, Private Bag 11008, Palmerston North, 4442, New Zealand; Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4410, New Zealand. Electronic address: [email protected] , (New Zealand)
  • 2 Food Nutrition & Health Team, AgResearch, Private Bag 11008, Palmerston North, 4442, New Zealand; Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4410, New Zealand. , (New Zealand)
  • 3 Fonterra Research and Development Centre, Fonterra Co-Operative Group, Private Bag 11029, Palmerston North 4442, New Zealand. , (New Zealand)
  • 4 Food Nutrition & Health Team, AgResearch, Private Bag 11008, Palmerston North, 4442, New Zealand. , (New Zealand)
  • 5 Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4410, New Zealand; Fonterra Research and Development Centre, Fonterra Co-Operative Group, Private Bag 11029, Palmerston North 4442, New Zealand. , (New Zealand)
  • 6 Food Nutrition & Health Team, AgResearch, Private Bag 11008, Palmerston North, 4442, New Zealand; Riddet Institute, Massey University, Private Bag 11222, Palmerston North 4410, New Zealand; High-Value Nutrition National Science Challenge, Auckland, New Zealand. , (New Zealand)
Type
Published Article
Journal
Journal of Dairy Science
Publisher
American Dairy Science Association
Publication Date
Dec 01, 2019
Volume
102
Issue
12
Pages
10772–10778
Identifiers
DOI: 10.3168/jds.2019-16951
PMID: 31629525
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Colostrum plays an important role in initiating the development of the intestinal barrier in newborn mammals. Given its bioactivity, there is much interest in the potential use of bovine colostrum to improve human gastrointestinal health throughout the life span. There is evidence that bovine colostrum is effective at improving small intestinal barrier integrity and some indication that it may alter colonic motility. However, for colostrum to be used as a product to improve intestinal health, it needs to be bioactive after processing. The aim of this study was to determine whether industrial processing of bovine colostrum affects its ability to improve small intestinal barrier integrity or alter distal colon motility. Three colostrum sample types were compared; raw whole colostrum powder (WCP), raw skim colostrum powder (SCP), and industrially produced colostrum milk protein concentrate (CMPC). To determine whether these colostrum powders had different effects on small intestinal barrier integrity, their effects on the transepithelial electrical resistance across an in vitro intestinal epithelial layer (Caco-2 cells) were measured, both with and without a challenge from the proinflammatory cytokine tumor necrosis factor-α. These results showed that CMPC enhanced transepithelial electrical resistance across unchallenged epithelial cell layers, whereas the raw colostrum samples, WCP and SCP, did not have an effect. The colostrum samples were also compared to determine how they affect contractility in the distal colon isolated from the rat. Skim colostrum powder was the only sample to act directly on colonic tissue to modulate motility, increasing the amplitude of contractions. The results show that bovine colostrum is able to improve small intestinal barrier integrity and alter colon motility, and they implicate different components. The barrier integrity enhancement was apparent only in the industrial CMPC, which may have been due to the increase in protein concentration or the release of small peptides as a result of processing. The ability to alter colon motility was present in SCP but absent in WCP, again implying that an increase in protein concentration is responsible for the effect. However, this effect was not apparent for the industrially processed CMPC, suggesting denaturation or degradation of the active component. The beneficial effect of colostrum on small intestinal barrier integrity was present after processing, confirming that it is feasible to industrially produce an active product for gut health. Copyright © 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

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