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Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

Authors
  • Teixeira da Silva, Jaime A.1, 2
  • Nezami-Alanagh, Esmaeil3, 4
  • Barreal, María E.3
  • Kher, Mafatlal M.5
  • Wicaksono, Adhityo6
  • Gulyás, Andrea2
  • Hidvégi, Norbert2
  • Magyar-Tábori, Katalin2
  • Mendler-Drienyovszki, Nóra2
  • Márton, László2
  • Landín, Mariana7
  • Gallego, Pedro Pablo3
  • Driver, John A.8
  • Dobránszki, Judit2
  • 1 1 , Ikenobe (Japan)
  • 2 Research Institute of Nyíregyháza, IAREF, University of Debrecen, Nyíregyháza, 4400, Hungary , Nyíregyháza (Hungary)
  • 3 University of Vigo, Vigo, 36310, Spain , Vigo (Spain)
  • 4 East Azarbaijan Science and Technology Park, Tabriz, Iran , Tabriz (Iran)
  • 5 GSFC University, Vadodara, Gujarat, 391750, India , Vadodara (India)
  • 6 Generasi Biologi Indonesia (Genbinesia) Foundation, Jl. Swadaya Barat No. 4, Gresik Regency, 61171, Indonesia , Gresik Regency (Indonesia)
  • 7 University of Santiago, Santiago de Compostela, Spain , Santiago de Compostela (Spain)
  • 8 Driver Consulting Inc., 2601 Tim Bell Road, Waterford, CA, 95386, USA , Waterford (United States)
Type
Published Article
Journal
Planta
Publisher
Springer-Verlag
Publication Date
Sep 03, 2020
Volume
252
Issue
3
Identifiers
DOI: 10.1007/s00425-020-03449-4
Source
Springer Nature
Keywords
License
Green

Abstract

Main conclusionShoot tip necrosis is a physiological condition that negatively impacts the growth and development of in vitro plant shoot cultures across a wide range of species.AbstractShoot tip necrosis is a physiological condition and disorder that can arise in plantlets or shoots in vitro that results in death of the shoot tip. This condition, which can spread basipetally and affect the emergence of axillary shoots from buds lower down the stem, is due to the cessation of apical dominance. STN can occur at both shoot multiplication and rooting stages. One of the most common factors that cause STN is nutrient deficiency or imbalance. Moreover, the presence or absence of plant growth regulators (auxins or cytokinins) at specific developmental stages may impact STN. The cytokinin to auxin ratio within an in vitro plant can be modified by varying the concentration of cytokinins used in the culture medium. The supply of nutrients to in vitro shoots or plantlets might also affect their hormonal balance, thus modifying the occurrence of STN. High relative humidity within culture vessels and hyperhydricity are associated with STN. An adequate supply of calcium as the divalent cation (Ca2+) can hinder STN by inhibiting the accumulation of phenolic compounds and thus programmed cell death. Moreover, the level of Ca2+ affects auxin transport and ethylene production, and higher ethylene production, which can occur as a result of high relative humidity in or poor ventilation of the in vitro culture vessel, induces STN. High relative humidity can decrease the mobility of Ca2+ within a plant, resulting in Ca2+ deficiency and STN. STN of in vitro shoots or plantlets can be halted or reversed by altering the basal medium, mainly the concentration of Ca2+, adjusting the levels of auxins or cytokinins, or modifying culture conditions. This review examines the literature related to STN, seeks to discover the associated factors and relations between them, proposes practical solutions, and attempts to better understand the mechanism(s) underlying this condition in vitro.

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