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Sheathless electrospray from polymer microchips.

Authors
  • Svedberg, Malin
  • Pettersson, Andreas
  • Nilsson, Stefan
  • Bergquist, Jonas
  • Nyholm, Leif
  • Nikolajeff, Fredrik
  • Markides, Karin
Type
Published Article
Journal
Analytical chemistry
Publication Date
Aug 01, 2003
Volume
75
Issue
15
Pages
3934–3940
Identifiers
PMID: 14572066
Source
Medline
License
Unknown

Abstract

In this study, sheathless electrospray from polymer microchips with conducting layer on the emitter tip is described for the first time. The electrospray emitter tips were fabricated directly from the end of the microchips that were made of polycarbonate or poly(methyl methacrylate) with injection molding. A variety of tip shapes and conducting coatings were evaluated using an electrospray time-of-flight mass spectrometer run in the sheathless mode. Stable electrospray was obtained both from hand-polished and machine-milled three-dimensional tips coated with either polymer-embedded gold particles or graphite particles as the conducting layer. Sputtered gold, on the other hand, suffered from poor stability mainly due to bad adhesion to the polymer tip. The durability of the different coatings was confirmed with electrochemical experiments under simulated electrospray conditions. The relative standard deviations of the response received from the ion current of the MS analysis were in the range of 3.5-12%. The detection limit for a standard mixture containing five neuropeptides was lower than 0.5 fmol. The low detection limit makes the emitter tips highly attractive for the analysis of low-abundance biological species.

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