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SGRL can regulate chlorophyll metabolism and contributes to normal plant growth and development in Pisum sativum L.

Authors
  • Bell, Andrew1
  • Moreau, Carol1
  • Chinoy, Catherine1
  • Spanner, Rebecca1
  • Dalmais, Marion2
  • Le Signor, Christine3
  • Bendahmane, Abdel2
  • Klenell, Markus4
  • Domoney, Claire5
  • 1 John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
  • 2 INRA/CNRS - URGV, 2 rue Gaston Crémieux, 91057, Evry, France. , (France)
  • 3 INRA, UMR 1347 Agroécologie, Dijon, France. , (France)
  • 4 School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK.
  • 5 John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK. [email protected]
Type
Published Article
Journal
Plant molecular biology
Publication Date
Dec 01, 2015
Volume
89
Issue
6
Pages
539–558
Identifiers
DOI: 10.1007/s11103-015-0372-4
PMID: 26346777
Source
Medline
Keywords
License
Unknown

Abstract

Among a set of genes in pea (Pisum sativum L.) that were induced under drought-stress growth conditions, one encoded a protein with significant similarity to a regulator of chlorophyll catabolism, SGR. This gene, SGRL, is distinct from SGR in genomic location, encoded carboxy-terminal motif, and expression through plant and seed development. Divergence of the two encoded proteins is associated with a loss of similarity in intron/exon gene structure. Transient expression of SGRL in leaves of Nicotiana benthamiana promoted the degradation of chlorophyll, in a manner that was distinct from that shown by SGR. Removal of a predicted transmembrane domain from SGRL reduced its activity in transient expression assays, although variants with and without this domain reduced SGR-induced chlorophyll degradation, indicating that the effects of the two proteins are not additive. The combined data suggest that the function of SGRL during growth and development is in chlorophyll re-cycling, and its mode of action is distinct from that of SGR. Studies of pea sgrL mutants revealed that plants had significantly lower stature and yield, a likely consequence of reduced photosynthetic efficiencies in mutant compared with control plants under conditions of high light intensity.

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