Affordable Access

Access to the full text

SGLT1 Deficiency Turns Listeria Infection into a Lethal Disease in Mice

Authors
  • Sharma, Piyush
  • Khairnar, Vishal
  • Madunić, Ivana Vrhovac
  • Singh, Yogesh
  • Pandyra, Aleksandra
  • Salker, Madhuri S.
  • Koepsell, Hermann
  • Sabolić, Ivan
  • Lang, Florian
  • Lang, Philipp A.
  • Lang, Karl S.
Type
Published Article
Journal
Cellular Physiology and Biochemistry
Publisher
S. Karger AG
Publication Date
Jul 14, 2017
Volume
42
Issue
4
Pages
1358–1365
Identifiers
DOI: 10.1159/000479197
PMID: 28704812
Source
Karger
Keywords
License
Green
External links

Abstract

Background: Cellular glucose uptake may involve either non-concentrative glucose carriers of the GLUT family or Na+-coupled glucose-carrier SGLT1, which accumulates glucose against glucose gradients and may thus accomplish cellular glucose uptake even at dramatically decreased extracellular glucose concentrations. SGLT1 is not only expressed in epithelia but as well in tumour cells and immune cells. Immune cell functions strongly depend on their metabolism, therefore we hypothesized that deficiency of SGLT1 modulates the defence against bacterial infection. To test this hypothesis, we infected wild type mice and gene targeted mice lacking functional SGLT1 with Listeria monocytogenes. Methods: SGLT1 deficient mice and wild type littermates were infected with 1x104 CFU Listeria monocytogenes intravenously. Bacterial titers were determined by colony forming assay, SGLT1, TNF-α, IL-6 and IL-12a transcript levels were determined by qRT-PCR, as well as SGLT1 protein abundance and localization by immunohistochemistry. Results: Genetic knockout of SGLT1 (Slc5a1–/– mice) significantly compromised bacterial clearance following Listeria monocytogenes infection with significantly enhanced bacterial load in liver, spleen, kidney and lung, and significantly augmented hepatic expression of TNF-α and IL-12a. While all wild type mice survived, all SGLT1 deficient mice died from the infection. Conclusions: SGLT1 is required for bacterial clearance and host survival following murine Listeria infection.

Report this publication

Statistics

Seen <100 times