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Severity of the Streptomycin Resistance and Streptomycin Dependence Phenotypes of Ribosomal Protein S12 of Thermus thermophilus Depends on the Identity of Highly Conserved Amino Acid Residues

Authors
  • Jennifer F. Carr
  • Steven T. Gregory
  • Albert E. Dahlberg
Publisher
American Society for Microbiology
Publication Date
May 01, 2005
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

The structural basis for the streptomycin dependence phenotype of ribosomal protein S12 mutants is poorly understood. Here we describe the application of site-directed mutagenesis and gene replacement of Thermus thermophilus rpsL to assess the importance of side chain identity and tertiary interactions as phenotypic determinants of drug-dependent mutants.

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