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Seroprevalence of fasciolosis in Hokkaido sika deer (Cervus nippon yesoensis) from Hokkaido Prefecture, Japan revealed by ELISA using recombinant cathepsin L1.

Authors
  • Sato, Hironobu1
  • Hiraya, Hiroki2
  • Sugiyama, Takutoshi1
  • Fukumoto, Shinya3
  • Matsuyama, Ryota4
  • Yanagawa, Yojiro5
  • Nakao, Ryo6
  • Irie, Takao7
  • Taira, Kensuke8
  • Yamazaki, Akiko2
  • Hagiwara, Katsuro9
  • Yoshida, Ayako10
  • Kamata, Yoichi11
  • Ichikawa-Seki, Madoka12
  • 1 Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan. , (Japan)
  • 2 Laboratory of Veterinary Public health, Faculty of Agriculture, Iwate University, Morioka, Japan. , (Japan)
  • 3 National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. , (Japan)
  • 4 Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. , (Japan)
  • 5 Laboratory of Theriogenology, Department of Clinical Sciences, Division of Veterinary Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. , (Japan)
  • 6 Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. , (Japan)
  • 7 Department of Infection Diseases, Hokkaido Institute of Public Health, Sapporo, Japan; Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan. , (Japan)
  • 8 Laboratory of Parasitology, School of Veterinary Medicine, Azabu University, Sagamihara, Japan. , (Japan)
  • 9 School of Veterinary Medicine, Rakuno-Gakuen University, Ebetsu, Japan. , (Japan)
  • 10 Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Centre for Animal Diseases Control (CADIC), University of Miyazaki, Miyazaki, Japan. , (Japan)
  • 11 Laboratory of Veterinary Public health, Faculty of Agriculture, Iwate University, Morioka, Japan; Department of Food and Nutrition, Faculty of Human Life Science, Senri Kinran University, Suita, Japan. , (Japan)
  • 12 Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Morioka, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Parasitology international
Publication Date
Feb 01, 2021
Volume
80
Pages
102222–102222
Identifiers
DOI: 10.1016/j.parint.2020.102222
PMID: 33137508
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Fasciolosis, a zoonotic disease caused by liver flukes of the genus Fasciola, has been reported in Hokkaido (Yezo) sika deer (Cervus nippon yesoensis) in Hokkaido Prefecture, Japan; however, the actual seroprevalence in the animal has not been adequately evaluated. The objective of the present study was to analyze the seroprevalence of the disease among Hokkaido sika deer. Recombinant cathepsin L1 (rCatL1) was used as an antigen for an indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola flukes. The sensitivity and specificity of the ELISA were 84.6% and 100%, respectively. The average seroprevalence in 1109 Hokkaido sika deer from 20 locations in Hokkaido Prefecture was 43.9%. Mature deer showed higher seroprevalence than younger individuals; however, even younger animals may act as a reservoir for the disease. Monitoring infection levels in the Hokkaido sika deer population is important not only for the livestock industry, but also for preventing human fasciolosis. Copyright © 2020 Elsevier B.V. All rights reserved.

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