Affordable Access

Access to the full text

Serodiagnostic evaluation of recombinant CdtB of S. Typhi as a potential candidate for acute typhoid

  • Sharma, Tarang1
  • Sharma, Chandresh1
  • Sankhyan, Anurag1, 2
  • Bedi, Sanjiv Pal3
  • Bhatnagar, Shinjini4
  • Khanna, Navin5
  • Gautam, Vikas6
  • Sethi, Sunil6
  • Vrati, Sudhanshu7
  • Tiwari, Ashutosh1, 3
  • 1 Translational Health Science and Technology Institute, Centre for Bio-design & Diagnostics, Faridabad, Haryana, India , Faridabad (India)
  • 2 Central Research Institute, Kasauli, Himachal Pradesh, India , Kasauli (India)
  • 3 Postgraduate Institute of Medical Education and Research, Department of Experimental Medicine & Biotechnology, Chandigarh, India , Chandigarh (India)
  • 4 Translational Health Science and Technology Institute, Paediatric Biology Centre, Faridabad, Haryana, India , Faridabad (India)
  • 5 International Centre for Genetic Engineering & Biotechnology, New Delhi, India , New Delhi (India)
  • 6 Postgraduate Institute of Medical Education and Research, Department of Medical Microbiology, Chandigarh, India , Chandigarh (India)
  • 7 Regional Centre for Biotechnology, Faridabad, Haryana, India , Faridabad (India)
Published Article
Immunologic Research
Springer US
Publication Date
Jun 21, 2018
DOI: 10.1007/s12026-018-9009-4
Springer Nature


Typhoid fever caused by human restricted Salmonella typhi presents a considerable health burden on developing South-Asian nations like India. The suboptimal sensitivity and specificity associated with culture-based isolation of etiological agent and the extensively used surface antigen-based serological assays often lead to misdiagnosis and inappropriate antimicrobial treatment. The increasing reports of the emergence of resistant strains and undefined disease burden signify the critical need for an inexpensive, reliable, easy-to-use, and highly sensitive diagnostic test for typhoid fever. Utilizing S. typhi-specific and immunogenic antigens in sero-diagnostic assays could lead to precise diagnosis of acute typhoid and prompt treatment. In this study, we report cloning, expression, and purification of recombinant Cytolethal distending toxin subunit B (CdtB) of S. typhi, which is reported to be highly specific, immunogenic, and expressed only upon S. typhi infection. We further evaluated the purified recombinant CdtB for its diagnostic potential in an IgM-based indirect ELISA format using 33 human samples. Twenty-one serum samples from blood culture confirmed cases (n = 21) of typhoid and 12 samples from healthy controls (n = 12) were tested. The assay showed sensitivity of 100% and specificity of 83.3% respectively with positive and negative predictive values of 91.3 and 100% respectively. Efficient detection of specific IgM antibodies indicates that CdtB could be highly valuable in sero-diagnosis of acute typhoid and rapid screening of clinical samples.

Report this publication


Seen <100 times