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Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication.

Authors
  • Salzman, N P
  • Sebring, E D
Type
Published Article
Journal
Journal of Virology
Publisher
American Society for Microbiology
Publication Date
Feb 01, 1967
Volume
1
Issue
1
Pages
16–23
Identifiers
PMID: 4248263
Source
Medline
Language
English
License
Unknown

Abstract

In vaccinia virus-infected cell cultures, cellular protein synthesis was inhibited 50% at 2 hr postinfection (PI) and 80 to 90% by 4 hr PI. Input virus was responsible for this inhibition. Five early proteins, coded for by the viral genome, could be detected at 2 to 3 hr PI. Normally, their synthesis did not continue beyond 6 hr PI, at which time synthesis of a different set of proteins began. When DNA replication was blocked, synthesis of these early proteins continued until 9 to 12 hr PI. The bulk of the proteins which were incorporated into mature virus were synthesized at 8 hr PI and thereafter. The time of their formation was close to the time at which virus maturation occurred. However, 15% of the protein found in mature virus was synthesized early in the infectious cycle. The quantity of "early viral protein" which was not incorporated into mature virus was almost as large as the quantity of viral protein which did appear in mature virus. The "early" and "late" proteins could be shown to have separate and distinct immunological properties. The role of this large quantity of "early" protein is discussed.

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