Affordable Access

Access to the full text

Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq

Authors
  • Alhatami, Abdullah O.1
  • Alaraji, Furkan2
  • Abdulwahab, Husam Muhsen2
  • Khudhair, Yahia Ismail3
  • 1 Department of Microbiology, Faculty of Veterinary Medicine, University of Kufa, Iraq
  • 2 Department of Pathology and Poultry Diseases, Faculty of Veterinary Medicine, University of Kufa, Iraq
  • 3 Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
Type
Published Article
Journal
Veterinary World
Publisher
Veterinary World
Publication Date
Jul 16, 2020
Volume
13
Issue
7
Pages
1358–1362
Identifiers
DOI: 10.14202/vetworld.2020.1358-1362
PMID: 32848311
PMCID: PMC7429401
Source
PubMed Central
Keywords
License
Green

Abstract

Background and Aim: Infectious bronchitis (IB) has an influential economic impact on the poultry industry, causing huge losses each year due to the condemnation of infected chickens. Despite the use of many kinds of vaccines in Iraq, it is common to find IB problems in vaccinated chickens. Information about the strains that affect Iraqi chickens is very limited. Therefore, we aimed to detect the currently circulating strains of IB virus that cause frequent outbreaks in egg layers despite the use of vaccination against the virus. Materials and Methods: Isolate detection, sequencing, and phylogenetic analysis were performed using a rapid IB virus antigen kit (32 tracheal swabs), flinders technology associates (FTA) card (32 tracheal swabs), and partial gene sequencing (16 positive FTA samples). Results: The isolated strain was different from other strains, especially the strain isolated in the North of Iraq (Sulemania Strain) and shares 98% homology with an Israeli strain (Israel variant 2, IS 1494). Conclusion: Although more studies are needed to detect IB virus strains circulating in Iraq, this work lays the foundation for making a good strategy to control the disease and selecting vaccines that should be used in farms.

Report this publication

Statistics

Seen <100 times