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Sequence and structure of a serine transfer RNA with GCU anticodon from mosquito mitochondria.

Authors
Type
Published Article
Journal
Journal of molecular biology
Publication Date
Volume
176
Issue
2
Pages
251–260
Identifiers
PMID: 6565045
Source
Medline
License
Unknown

Abstract

We have determined the primary sequence and modification status of a transfer RNA from mosquito mitochondria whose GCU anticodon indicates that it is a serine tRNA (tRNASerGCU), and have obtained information on higher order structure using partial digestion with nucleases S1 and T1 under non-denaturing conditions. Although its primary sequence homology to mammalian mitochondrial tRNASerGCU is modest (46%), the mosquito tRNA resembles its mammalian mitochondrial counterpart in that a plausible secondary structure configuration includes a drastically abbreviated D arm and a sex base-pair anticodon stem. Other unusual features include a ribose-methylated cytidine residue at the end of the anticodon stem, and the likely occurrence of a psi residue between the amino acid arm and arm IV.

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