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Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide.

Authors
  • Nielsen, P E
  • Egholm, M
  • Berg, R H
  • Buchardt, O
Type
Published Article
Journal
Science (New York, N.Y.)
Publication Date
Dec 06, 1991
Volume
254
Issue
5037
Pages
1497–1500
Identifiers
PMID: 1962210
Source
Medline
License
Unknown

Abstract

A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.

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