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Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction.

Authors
  • Khodakov, Dmitriy A
  • Khodakova, Anastasia S
  • Linacre, Adrian
  • Ellis, Amanda V
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Jul 21, 2014
Volume
139
Issue
14
Pages
3548–3551
Identifiers
DOI: 10.1039/c4an00694a
PMID: 24888345
Source
Medline
License
Unknown

Abstract

This paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.

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