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The sequence of porcine alpha s1-casein cDNA: evidence for protein variants generated by altered RNA splicing.

Authors
  • Alexander, L J
  • Beattie, C W
Type
Published Article
Journal
Animal genetics
Publication Date
Jan 01, 1992
Volume
23
Issue
3
Pages
283–288
Identifiers
PMID: 1503265
Source
Medline
License
Unknown

Abstract

A cDNA library was constructed from mRNA isolated from lactating porcine mammary gland and screened with a bovine alpha s1-casein cDNA clone. Three classes of cDNA isolated varied in the number of bases within the coding region. The full length porcine alpha s1-casein cDNA is 1124bp and codes a preprotein of 206 amino acids. The other two classes of alpha s1-casein cDNA lacked 18bp and 60bp respectively when compared to the 1124-bp cDNA sequence. PCR amplification confirmed the presence of these sequences in total RNA. These differences appear to be due to altered RNA splicing.

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