Affordable Access

deepdyve-link
Publisher Website

Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes.

Authors
  • Huercha,1, 2
  • Ma, Ying2
  • Hao, Yunwei2
  • Li, Min2
  • Hu, Zhengxiang3
  • Song, Ruiqi1, 2
  • Wei, Liting2
  • Fan, Shilong2
  • Chen, Songqin2
  • Fan, Xinli2
  • Zhai, Xuejie2
  • Guo, Qingyong4
  • Bayin, Chahan5
  • 1 College of Animal Science, Xinjiang Agricultural University, Ürümqi, 830052, Xinjiang, China. , (China)
  • 2 College of Veterinary Medicine, Xinjiang Agricultural University, No.311 Nongda Road, Ürümqi, 830052, Xinjiang, China. , (China)
  • 3 Bayingol Vocational and Technical College, Korla, 841000, Xinjiang, China. , (China)
  • 4 College of Veterinary Medicine, Xinjiang Agricultural University, No.311 Nongda Road, Ürümqi, 830052, Xinjiang, China. [email protected] , (China)
  • 5 College of Veterinary Medicine, Xinjiang Agricultural University, No.311 Nongda Road, Ürümqi, 830052, Xinjiang, China. [email protected] , (China)
Type
Published Article
Journal
Experimental and Applied Acarology
Publisher
Springer-Verlag
Publication Date
Sep 29, 2020
Identifiers
DOI: 10.1007/s10493-020-00546-7
PMID: 32995924
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Dermacentor marginatus is a widespread tick species and a vector of many pathogens in Eurasia. Due to the medical importance of D. marginatus, control measures are needed for this tick species. Currently tick control approaches rely mostly on acaricide application, whereas wrong and irrational acaricide use may result in drug resistance and residue problems. Vaccination as an alternative approach for tick control has been proven to be effective towards some tick species. However, immunization against D. marginatus has not yet reached satisfactory protection. The effort of in silico based analysis could predict antigenicity and identify candidates for anti-tick vaccine development. We carried out an in silico analysis of D. marginatus glutathione S-transferases (DmGSTs) in order to identify blood-feeding induced GSTs as antigens that can be used in anti-tick vaccine development. Phylogenetic analysis, linear B-cell epitope prediction, homology modeling, and conformational B-cell epitope mapping on the GST models were performed to identify highly antigenic DmGSTs. Relative gene expressions of the seven GSTs were profiled through real-time quantitative PCR (RT-qPCR) to outline GSTs up-regulated during blood feeding. The phylogenetic analysis indicated that the seven GSTs belonged to four classes of GST, including one in epsilon-class, one in zeta-class, one in omega-class, and four in mu-class. Linear B-cell epitope prediction revealed mu-class GSTs share similar conserved antigenic regions. The conformational B-cell epitope mapped on the homology model of the GSTs displayed that GSTs of mu-class showed stronger antigenicity than that of other classes. RT-qPCR revealed DmGSTM1 and DmGSTM2 were positively related to blood feeding. In sum, the data suggest that DmGSTM1 and DmGSTM2 could be tested for potential anti-tick vaccine trials.

Report this publication

Statistics

Seen <100 times