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Separation, identification and determination of sanguinarine in argemone and other adulterated edible oils by reversed-phase high-performance liquid chromatography.

Authors
  • Husain, S
  • Narsimha, R
  • Rao, R N
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Nov 19, 1999
Volume
863
Issue
1
Pages
123–126
Identifiers
PMID: 10591470
Source
Medline
License
Unknown

Abstract

A simple, rapid and reliable reversed-phase high-performance liquid chromatographic method for the separation and determination of sanguinarine in argemone and other edible oils has been developed. The separation has been achieved on a C18 column with CH3OH-CH3CN-tetrahydrofuran-water as mobile phase using diode array detection at 280 nm. The minimum detection limit of sanguinarine in the adulterated edible oils is 5 microg/g.

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