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Separation of free and protein-bound ligands in the radioimmunoassay by gel filtration-centrifugation.

Authors
  • Fránek, M
  • Hruska, K J
Type
Published Article
Journal
Journal of chromatography
Publication Date
Apr 28, 1976
Volume
119
Pages
167–172
Identifiers
PMID: 1262416
Source
Medline
License
Unknown

Abstract

The application of gel filtration-centrifugation to the separation of free and protein-bound ligands in radioimmunoassay is described. The method is simple and rapid. A large number of samples can be handled simultaneously and the bount radioactivity is directly transferred to the scintillation vial. Using a 9 X 36 mm column of Sephadex G-50 (coarse), 400 mul of the reaction mixture can be separated during 2 min centrifugation.

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