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Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.

Authors
  • Lewis, F A
  • Griffiths, S
  • Dunnicliff, R
  • Wells, M
  • Dudding, N
  • Bird, C C
Type
Published Article
Journal
Journal of clinical pathology
Publication Date
Feb 01, 1987
Volume
40
Issue
2
Pages
163–166
Identifiers
PMID: 3029184
Source
Medline
License
Unknown

Abstract

A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.

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