Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
- Published Article
Journal of clinical pathology
- Publication Date
Feb 01, 1987
A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/3029184