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A sensitive fluorescence monitor for the detection of activated Ras: total chemical synthesis of site-specifically labeled Ras binding domain of c-Raf1 immobilized on a surface.

Authors
  • Becker, C F
  • Hunter, C L
  • Seidel, R P
  • Kent, S B
  • Goody, R S
  • Engelhard, M
Type
Published Article
Journal
Chemistry & Biology
Publisher
Elsevier
Publication Date
Mar 01, 2001
Volume
8
Issue
3
Pages
243–252
Identifiers
PMID: 11306349
Source
Medline
License
Unknown

Abstract

The site-specific incorporation of a fluorescent group at a strategic position in a Ras effector protein allows the detection of activated Ras with high sensitivity. This example illustrates the fact that the chemical synthesis of proteins or protein domains makes it possible to incorporate any kind of natural or unnatural amino acid at the position of choice, thereby enabling the facile preparation of specific biosensors, enhanced detection systems for drug screening, or the synthesis of activated proteins, e.g. phosphorylated proteins involved in signaling pathways, as defined molecular species.

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