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A sensitive flow injection chemiluminescence method for the determination of progesterone.

Authors
Type
Published Article
Journal
Drug Testing and Analysis
1942-7611
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
5
Issue
4
Pages
242–246
Identifiers
DOI: 10.1002/dta.299
PMID: 21671425
Source
Medline
License
Unknown

Abstract

A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II) - potassium permanganate (KMnO4 ) - progesterone in acidic medium sensitized by Na2 SO3 . With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10(-10) ∼ 6.0 × 10(-9) g·ml(-1) and 6.0 × 10(-9) ∼ 4.0 × 10(-8) g·ml(-1) with a detection limit of 7.1 × 10(-11) g·ml(-1) . The relative standard deviation (RSD) was 2.79% for 1.0 × 10(-8) g·ml(-1) progesterone (n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed.

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