A sensitive flow injection chemiluminescence method for the determination of progesterone.
- Authors
- Type
- Published Article
- Journal
- Drug Testing and Analysis
- Publisher
- Wiley (John Wiley & Sons)
- Publication Date
- Apr 01, 2013
- Volume
- 5
- Issue
- 4
- Pages
- 242–246
- Identifiers
- DOI: 10.1002/dta.299
- PMID: 21671425
- Source
- Medline
- License
- Unknown
Abstract
A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II) - potassium permanganate (KMnO4 ) - progesterone in acidic medium sensitized by Na2 SO3 . With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10(-10) ∼ 6.0 × 10(-9) g·ml(-1) and 6.0 × 10(-9) ∼ 4.0 × 10(-8) g·ml(-1) with a detection limit of 7.1 × 10(-11) g·ml(-1) . The relative standard deviation (RSD) was 2.79% for 1.0 × 10(-8) g·ml(-1) progesterone (n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed.