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A Semisynthetic Fluorescent Sensor Protein for Glutamate

Authors
  • Brun, Matthias A.
  • Tan, Kui-Thong
  • Griss, Rudolf
  • Kielkowska, Anna
  • Reymond, Luc
  • Johnsson, Kai
Publication Date
Jan 01, 2012
Source
Infoscience @ EPFL
Keywords
License
Unknown
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Abstract

We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.

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