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Semisynthetic cytochrome c.

Authors
  • Le, Barstow
  • Rs, Young
  • E, Yakali
  • Jj, Sharp
  • Jc, O Brien
  • Phil Berman
  • Ha, Harbury
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Volume
74
Issue
10
Pages
4248–4250
Source
UCSC Bioinformatics biomedical-ucsc
License
Unknown

Abstract

Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.

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