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A semiquantitative probe for radiation-induced normal tissue damage at the molecular level.

Authors
  • Miller, G
  • Siemann, D
  • Scott, P
  • Dawson, D
  • Muldrew, K
  • Trépanier, P
  • McGann, L
Type
Published Article
Journal
Radiation research
Publication Date
Jan 01, 1986
Volume
105
Issue
1
Pages
76–83
Identifiers
PMID: 3511495
Source
Medline
License
Unknown

Abstract

Sheep antibodies to bovine type I collagen were employed in the immunohistochemical detection of type I collagen in lung tissue sections of irradiated LAF1 mice. A video image digitizing system was developed to estimate collagen levels, by assigning a numerical value (0-63) to each of approximately 53,800 picture elements (pixels) in the microscope field, according to the collagen-dependent fluorescence intensity at each locus. For lungs harvested 52 weeks subsequent to graded doses of 60Co gamma radiation between 0 and 10 Gy, a dose-dependent increase in type I collagen was observed in the alveolar walls. A reproducible increase was evident for doses as low as 5 Gy: doses of 7 to 10 Gy elicited type I collagen levels significantly elevated with respect to those of age-matched controls. These results are consistent with a role for type I collagen in the development of radiation-induced pulmonary fibrosis. The assay system developed here will be used to explore the role of connective tissue macromolecules in the development of radiation pneumonitis and fibrosis.

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