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Semi-quantitative Analysis of Tramadol, Dextromethorphan and Metabolites in Decomposed Skeletal Tissues by Ultra Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry (UPLC-qTOF-MS).

Authors
  • Cornthwaite, H M1
  • Labine, L2
  • Watterson, J H1, 2
  • 1 Department of Chemistry & Biochemistry, Laurentian University, 935 Ramsey Lake Rd, Sudbury, Ontario, Canada, P3E 2C6. , (Canada)
  • 2 Department of Forensic Science, Laurentian University, 935 Ramsey Lake Rd, Sudbury, Ontario, Canada, P3E 2C6. , (Canada)
Type
Published Article
Journal
Drug Testing and Analysis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Oct 20, 2017
Identifiers
DOI: 10.1002/dta.2327
PMID: 29055108
Source
Medline
Keywords
License
Unknown

Abstract

The use of filtration/pass-through extraction (FPTE) and ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) to detect tramadol (TRAM), dextromethorphan (DXM) and metabolites from skeletal remains is described. Rats (n=5) received 50 mg/kg tramadol and were euthanized by CO2 asphyxiation approximately 30 min post-dose. Rats (n=4) received 75 mg/kg dextromethorphan and were euthanized by CO2 asphyxiation approximately 45 min post-dose. Remains decomposed to skeleton outdoors and vertebral bones were collected. Bones were cleaned, dried and pulverized to a fine powder. Bone underwent dynamic methanolic extraction followed by FPTE before analysis using UPLC-qTOF-MS. Recovery was at least 90% of maximal value within the first 10 min of methanolic extraction for all samples assayed. Analytical response was measured over the concentration range of 1-500 ng/mL, with precision and bias <20% in triplicate analyses of all calibrators, and a limit of detection of 1 ng/mL for TRAM, DXM and all metabolites. The vertebral bone analyzed using this method detected TRAM, DXM and their respective metabolites in all samples analyzed.

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