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A Semi-automated and Scalable 3D Spheroid Assay to Study Neuroblast Migration

Authors
  • Ducker, Martin1
  • Millar, Valerie2
  • Ebner, Daniel2
  • Szele, Francis G.1
  • 1 Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK
  • 2 Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK
Type
Published Article
Journal
Stem Cell Reports
Publisher
Elsevier
Publication Date
Aug 06, 2020
Volume
15
Issue
3
Pages
789–802
Identifiers
DOI: 10.1016/j.stemcr.2020.07.012
PMID: 32763162
PMCID: PMC7486343
Source
PubMed Central
Keywords
License
Unknown

Abstract

Cell migration is essential in development and regeneration and contributes to cancer pathology. Here, Szele and colleagues have developed and used a fast, easy, and robust assay for neuroblast migration. The assay is versatile, with medium throughput and was used to screen a kinase inhibitor library. They found and validated that MuSK and PIK3CB are necessary for neuroblast migration.

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