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A self-gelling hydrogel based on thiolated hyaluronic acid for three-dimensional culture of ovine preantral follicles.

Authors
  • Dahmardeh, Tayebeh1
  • Ghanian, Mohammad Hossein2
  • Ebrahimi, Bita3
  • 1 Department of Reproductive Biology, Faculty of Basic Science and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. , (Iran)
  • 2 Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: [email protected]. , (Iran)
  • 3 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Electronic address: [email protected]. , (Iran)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Dec 31, 2023
Volume
253
Issue
Pt 5
Pages
127147–127147
Identifiers
DOI: 10.1016/j.ijbiomac.2023.127147
PMID: 37778594
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Three-dimensional (3D) ovarian follicle culture offers a promising option for fertility preservation in patients who cannot receive ovarian tissue transplantation. Our research evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to routinely used alginate hydrogel (ALG). Synthesized via a carbodiimide reaction, HA-SH facilitated a self-crosslinking hydrogel through disulfide bond formation. Ovine preantral follicles (200-300 μm) retrieved through mechanical and enzymatic methods were encapsulated individually in either ALG or HA-SH hydrogels. Although both hydrogels adequately supported follicle survival, 3D integrity, and antrum formation over a 17-day in vitro culture, follicle growth was significantly higher within the HA-SH hydrogel. Gene expression analysis underscored that some folliculogenesis-related genes (ZP3, BMP7, and GJA1) and a steroidogenic gene (CYP19A1) demonstrated higher expression levels in HA-SH encapsulated follicles versus ALG. Collectively, our findings advocate for HA-SH hydrogel as a potent biomaterial for in vitro follicle cultures, attributing its efficacy to facile gelation, bio-responsiveness, and superior support for follicle growth. Copyright © 2023 Elsevier B.V. All rights reserved.

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